It's important that you do not retail store your HPLC column even though it still is made up of any buffers or ion-pairing agents. To guarantee this, flush five column quantityDisconnect the road main through the column to the detector. This can avert any flushed contaminants from finding lodged in the latter factors of your system. I usually let t
Third-party experience may not Examine towards the deep know-how and comprehensive training of an Agilent-Licensed support Qualified.Know your column: Columns have distinct pore sizes and solvent tolerances. Examine Along with the producer in the column to stop loading incompatible samples and solvents.but changing the buffer with HPLC grade water.
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